Oxygen tension in isolated transplanted rat islets and in islets of rat whole-pancreas transplants
نویسندگان
چکیده
منابع مشابه
Markedly decreased oxygen tension in transplanted rat pancreatic islets irrespective of the implantation site.
In this study, we syngeneically transplanted islets to three different implantation sites of diabetic and nondiabetic rats, then 9-12 weeks later we measured the blood perfusion and compared the tissue partial pressure of oxygen (PO2) levels of these transplanted islets to endogenous islets. Modified Clark microelectrodes (outer tip diameter 2-6 microm) were used for the oxygen tension measurem...
متن کاملMONOPHASIC STATE OF INSULIN SECRETION IN ISOLATED RAT ISLETS OF LANGERHANS BY K+, Ca2+ AND GLUCOSE
Isolation of rat islets of Langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of Hanks' solution into the common bile duct. The identification of the islets was done by techniques including the use of a stereomicroscope against a black background. Vital staining was done by an injection of neutral red solution through t...
متن کاملEffect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets
Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containi...
متن کاملpH is decreased in transplanted rat pancreatic islets
Carlsson, Per-Ola, Astrid Nordin, and Fredrik Palm. pH is decreased in transplanted rat pancreatic islets. Am J Physiol Endocrinol Metab 284: E499–E504, 2003. First published November 5, 2002; 10.1152/ajpendo.00156.2002.—Recent studies of transplanted pancreatic islets have indicated incomplete revascularization. We investigated the pH, in relation to oxygen tension (PO2), in endogenous islets ...
متن کاملpH is decreased in transplanted rat pancreatic islets
Carlsson, Per-Ola, Astrid Nordin, and Fredrik Palm. pH is decreased in transplanted rat pancreatic islets. Am J Physiol Endocrinol Metab 284: E499–E504, 2003. First published November 5, 2002; 10.1152/ajpendo.00156.2002.—Recent studies of transplanted pancreatic islets have indicated incomplete revascularization. We investigated the pH, in relation to oxygen tension (PO2), in endogenous islets ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Transplant International
سال: 2002
ISSN: 0934-0874,1432-2277
DOI: 10.1111/j.1432-2277.2002.tb00112.x